Microscopy & Microtechnique

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Remember the basic units used in Microscopy.
- One millimeter = 1000 micrometer (mm).
- One mm = 1000 nanometer.
Microtechnique = tissue preparation for microscopic examination.
- It usually involves hardening of the tissue followed by sectioning (cutting). e.g Paraffin & freezing techniques
- The freezing technique is faster and preserve the chemical components of tissue.
- The Paraffin technique is slower but preserve the integrity of the cut sections.
Staining Techniques include:
- Ordinary stains: e.g. Hematoxylin & Eosin, Masson trichrome stain, orcein stain, silver stain and PAS technique.
- Histochemistry & Cytochemistry; Immunohistochemistry and in situ Hybridization Techniques.
There are two main types of Microscopies: Light and Electron
- Types of Light Microscopy include: Phase Contrast, Fluorescence, and Confocal
- Types of Electron Microscopy include Transmission and Scanning Electron Microscopy.